bhk-21 medium (Thermo Fisher)
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bhk-21 medium
Bhk 21 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bhk+21+medium/bhk+21+cells/pmc11666578-223-40-12
Average 90 stars, based on 1 article reviews
Bhk 21 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bhk+21+medium/bhk+21+cells/pmc11666578-223-40-12
Average 90 stars, based on 1 article reviews
bhk-21 medium - by Bioz Stars,
2026-10
90/100 stars
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Transfection:Article Title: Respiratory Syncytial Virus (RSV) Fusion Protein Subunit F2, Not Attachment Protein G, Determines the Specificity of RSV Infection Article Snippet: .. The transfection medium was replaced after 4 h with Article Title: Infectious RNA vaccine protects mice against chikungunya virus infection Article Snippet: Serum was diluted in minimum essential medium (MEM) containing 0.2% bovine serum albumin (BSA) (Gibco/Life Technologies) and tested by plaque assay as described previously . .. Briefly, confluent BHK-21 cells in 12-well plates were washed with PBS twice, serum or supernatants harvested from iRNA transfected BHK-21 cells in serial dilutions were added to the wells, and the wells were incubated at 37 °C in 5% CO 2 for 1 h. The infection medium was removed, and 1 ml of overlay medium, consisting of 1 part of 2% carboxymethyl cellulose (CMC, Avicel, Belgium) in PBS and 2 parts of complete Incubation:Article Title: Infectious RNA vaccine protects mice against chikungunya virus infection Article Snippet: Serum was diluted in minimum essential medium (MEM) containing 0.2% bovine serum albumin (BSA) (Gibco/Life Technologies) and tested by plaque assay as described previously . .. Briefly, confluent BHK-21 cells in 12-well plates were washed with PBS twice, serum or supernatants harvested from iRNA transfected BHK-21 cells in serial dilutions were added to the wells, and the wells were incubated at 37 °C in 5% CO 2 for 1 h. The infection medium was removed, and 1 ml of overlay medium, consisting of 1 part of 2% carboxymethyl cellulose (CMC, Avicel, Belgium) in PBS and 2 parts of complete Infection:Article Title: Infectious RNA vaccine protects mice against chikungunya virus infection Article Snippet: Serum was diluted in minimum essential medium (MEM) containing 0.2% bovine serum albumin (BSA) (Gibco/Life Technologies) and tested by plaque assay as described previously . .. Briefly, confluent BHK-21 cells in 12-well plates were washed with PBS twice, serum or supernatants harvested from iRNA transfected BHK-21 cells in serial dilutions were added to the wells, and the wells were incubated at 37 °C in 5% CO 2 for 1 h. The infection medium was removed, and 1 ml of overlay medium, consisting of 1 part of 2% carboxymethyl cellulose (CMC, Avicel, Belgium) in PBS and 2 parts of complete CMC:Article Title: Infectious RNA vaccine protects mice against chikungunya virus infection Article Snippet: Serum was diluted in minimum essential medium (MEM) containing 0.2% bovine serum albumin (BSA) (Gibco/Life Technologies) and tested by plaque assay as described previously . .. Briefly, confluent BHK-21 cells in 12-well plates were washed with PBS twice, serum or supernatants harvested from iRNA transfected BHK-21 cells in serial dilutions were added to the wells, and the wells were incubated at 37 °C in 5% CO 2 for 1 h. The infection medium was removed, and 1 ml of overlay medium, consisting of 1 part of 2% carboxymethyl cellulose (CMC, Avicel, Belgium) in PBS and 2 parts of complete Cell Culture:Article Title: The Adjuvant Activity of Alphavirus Replicons Is Enhanced by Incorporating the Microbial Molecule Flagellin into the Replicon Article Snippet: .. BHK-21 cells were cultured in complete Article Title: Role of the Arf6 GDP/GTP Cycle and Arf6 GTPase-activating Proteins in Actin Remodeling and Intracellular Transport Article Snippet: .. Cell Culture and Antibodies—Baby hamster kidney (BHK) cells were grown in Article Title: Development and validation of four Real-Time quantitative RT-PCRs specific for the positive or negative strands of a bisegmented dsRNA viral genome. Article Snippet: Four tagged quantitative Real-Time RT-PCRs (qRT-PCRs) were developed to quantify the positive and negative strands of segments A and B of the bisegmented double-stranded RNA (dsRNA) genome of infectious bursal disease virus (IBDV, family Birnaviridae, genus Avibirnavirus).. The qRT-PCRs were validated using single-stranded RNAs corresponding to each genomic strand (A+, B+, A−, B−).. Specific quantitation proved possible from 5 × 107 to 5 × 102 copies of the template per reaction, with excellent reproducibility and linearity. Article Title: Formation of infectious pancreatic necrosis virus-like particles following expression of segment A by recombinant semliki forest virus. Article Snippet: Segment A of the Sp strain (a Norwegian field isolate) of infectious pancreatic necrosis virus (IPNV) was amplified by reverse transcriptase polymerase chain reaction in two stages from RNA isolated from infected cells, and cloned into the Semliki Forest virus (SFV) expression vector pSFV1.. Expression and correct processing of IPNV proteins was confirmed by transfection of RNA transcribed from this plasmid into BHK cells.. This clone was then used to produce recombinant replication-defective SFV particles (rSFV) expressing the IPNV segment A. Immunofluorescence studies with conformation-dependent monoclonal antibodies to IPNV confirmed that the recombinant proteins produced after infection of the salmonid cell line CHSE-214 with such rSFV retain their antigenicity. |